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INSTITUTIONAL LEVEL BIOTECH HUB
North Lakhimpur College (Autonomous), Assam
Sponsored by:
Department of Biotechnology
Ministry of Science & Technology, Govt. of India
Institutional Level Biotech Hub was established at North Lakhimpur College, Assam during the session 2011-12 with the financial assistance received from the Department of Biotechnology, Ministry of Science & Technology, Govt. of India under NER-BPMC.
The major objectives of establishing the Institutional Level Biotech Hub at North Lakhimpur College are to provide basic laboratory facilities with necessary equipments to carry on biotechnological research for the benefit of all the stakeholders and also to bring awareness in Biotechnology.
Establishment of the DBT sponsored Institutional Level Biotech Hub at North Lakhimpur College in 2011 has helped the college to provide-
List of equipment available in Biotech Hub:
Equipments for molecular Biological activities:
Sl. No. |
Equipments with Model No. & Brand Name |
1 |
96 well Gradient Thermal Cycler, TC-5000, Techne |
2 |
Digital pH Meter, MK VI, Systronics |
3 |
Gel Documentation System, XS-D56-0MAUTO, UVITEC |
4 |
Desktop Computer with Printer, HP S1935a |
5 |
Midi Submarine System, Preparative Model, GeNei |
6 |
Electrophoresis Power Pack (Supply), BGPS 300/400, GeNei Merck |
7 |
Cooler Box, GeNei Merck |
8 |
Vortex Mixer, 11-160, GeNei Merck |
9 |
14000rpm Microcentrifuge, SLM-MCF-14K, GeNei |
10 |
Vertical Deepfreezer, BFS-150, Cellfrost |
11 |
Single Channel Micropipette (a set of 5 with stand), T2,T1000,T20,T10; Tarson |
12 |
Dry bath, LI-DB-120, GeNei Merck |
13 |
Microwave Oven, SHARP |
14 |
UV-VIS Spectrophotometer, Systronics |
15 |
Online UPS with battery & stand, 3KVA (2hr), ELNOVA |
16 |
Silent Generator, 25A5-C, Kirloskar |
Equipments for Plant tissue culture activities:
Sl. No. |
Name of Equipment |
1 |
Refrigerated Liquid Bath inner chamber made of S.S., size 450x300x300mm |
2 |
Incubator Bacteriological 19”x18”x18” S.S. Chamber |
3 |
Deep Freezer , Ultra Low cabinet 4 cu feet, ambient to -30 |
4 |
Tissue Culture Hood , size 1200x600x 600mm height at back , S.steel top |
5 |
B.O.D. Incubator [650(W)x580(B)x900(H)mm Automatic cyclic Timer Digital Humidity Controller, Automatic Voltage Stabilizers |
6 |
Shaking Incubator cum BOD incubator 635x550x550mm, Digital Speed meter, & Digital Temp indicator cum Controller with Stabilizers |
7 |
Laminar Flow Cabinet Horizontal (SS), 3′x 2′x 2’ UV Light, Transparent front door, HEPA filters & PRE filters , Steel Revolving chair, Cooling System ( 1.0 tone), Automatic Voltage Stabilizers |
8 |
Tissue Culture Rack with Florescence Tubes & 5-shelved, 0-24 h Timer, & Stabilizers |
9 |
Distillation Apparatus Glass, (Double Stage) Accessories – Flask Capacity 5Lit, Automatic Low water level cut off device. |
10 |
Hot Air Oven size 455x455x605mm built in air fan and digital temp. controller |
11 |
Rotary Flask Shaker (Platform size 55 x 55 cm) Holding capacity 36 flask of 100ml, Digital Speed meter, Automatic 0-60minute Timer |
12 |
Autoclave Vertical 350mmx550mm, Digital ON/ OFF mechanical device, Automatic 0-60minute Timer ss, Inner busket, Lifting device, Safety valve, Pressure release valve, Water level indicator. |
13 |
Serological Water Bath double walled SS Chamber Size 605x300x175mm with digital temp. Controller & High speed stirrer. |
14 |
Test Tube Rotator for 36 tubes |
15 |
Culture Vessels Magnetic stirring capacity 5 ltr built in Hot plate |
16 |
Tissue Culture Trionocular Microscope with Microphotographic camera kit |
17 |
Micropipettes multi channels Analogue model |
18 |
Electronic Digital Balance , Analytical – Capacity 100gm, Sensitivity 1mg |
19 |
LCD Projector “Sony”, Multimedia Projector – Remote control , Portable tripod plastic screen – 60″ x 60″ |
20 |
Computers (Leonova Flat model) with UPS Accessories – Laser jet Printer-1, Inkjet Printer (1No.), Scanner (1No.) |
21 |
Tissue Floatation Bath 200MMX70MM |
22 |
PH Meter (Digital) built in stabilizer Accessories – Combined glass electrodes Buffer solution Stand. |
23 |
Microprocessor based pH meter with accessories |
24 |
Water Deioniser – Four bed Digital Model capacity 100Lit/hr. |
25 |
Tissue Homogenizer , 10000 rpm with speed regulator |
26 |
Tissue Processing Unit with S.S. basket, 24 tissues capsules, with thermostatic controlled wax bath |
27 |
Research Micro Centrifuge (Table Top Model) with rotor head carrier for 1x18 (5ml) tubes |
28 |
Autoclave Portable 300mm x 300mm Doubled wall With swing nut type closing lid. Safety valve, Pressure release valve |
Major achievements of the Biotech Hub:
The hub is well equipped for carrying out research both on Plant Biotechnology and Molecular Biology. The following Research projects were conducted in the hub.
a) Plant regeneration by somatic embryogenesis in Azadirachta indica (Neem).
b) In vitro micropropagation of Aloe vera, Brahmi, Orchids and Banana.
c) Phytochemical Screening and GC-MS Analysis of Methanolic leaf Extract of an
Endemic Plant K. assamica.
d) GC-MS Analysis of Bioactive Compounds Present in Methanol Extract of Fruit of An
Endemic Plant Kayea assamica.
e) Identification of medicinally important bioactive compounds present in fruit of endemic
plant K. assamica for the conservation and sustainable use of the plant species.
f) Hepato-Protective Effect of Aqueous Extract of Seed, Leaf and Fruit of Jackfruit
(Artocarpus heterophyllus Lam.) Against CCl4 Induced Hepatotoxicity on Swiss
Albino Mice.
g) In vitro germination of Bhut jolokiya from khashi Hills, Meghalaya.
h) Training of Teachers & undergraduate students; arrangement of invited talk, awareness
/outreach Program, observation of National Science Day, etc.
i) Training of UG, PG students and research scholars on handling modern sophisticated
instruments and equipment in the domain Biotechnology research.
j) A number of outreach programs/ invited talks realized to propel sensitization of
Biotech Research.
k) More and more outstation academicians and researchers showing keen interest to
have association with our Biotech Hub.
l) Mentoring Biotech Laboratory at Panigaon and Gogamukh H.S. School under Bliss
Program of DBT, Govt. of India
m) Publication of annual News Bulletin “BIO-NEWS” in the month of April every year.
Future activities and proposed action plan:
Papers published/ Presented: