biotech hub

INSTITUTIONAL LEVEL BIOTECH HUB

North Lakhimpur College (Autonomous), Assam

 

Sponsored by:

Department of Biotechnology

Ministry of Science & Technology, Govt. of India

 

                       

Institutional Level Biotech Hub was established at North Lakhimpur College, Assam during the session 2011-12 with the financial assistance received from the Department of Biotechnology, Ministry of Science & Technology, Govt. of India under NER-BPMC.

The major objectives of establishing the Institutional Level Biotech Hub at North Lakhimpur College are to provide basic laboratory facilities with necessary equipments to carry on biotechnological research for the benefit of all the stakeholders and also to bring awareness in Biotechnology.

 

 

            Establishment of the DBT sponsored Institutional Level Biotech Hub at North Lakhimpur College in 2011 has helped the college to provide-

  • Basic laboratory facilities with necessary equipments to carry on biotechnological research to all the stakeholders.
  • The students with an overview of the finer aspects of biotechnology and molecular biology.
  • Hands on trainings to the teachers and students of the neighboring institutions
  • New information on biotechnology through the in-house workshops, invited talks and various awareness/ outreach programs.
  • Observation of National Science Day, World Environment Day, etc...

List of equipment available in Biotech Hub:

 

Equipments for molecular Biological activities:

 

Sl. No.

Equipments with Model No. & Brand Name

1

96 well Gradient Thermal Cycler, TC-5000, Techne

2

Digital pH Meter, MK VI, Systronics

3

Gel Documentation System, XS-D56-0MAUTO, UVITEC

4

Desktop Computer with Printer, HP S1935a

5

Midi Submarine System, Preparative Model, GeNei

6

Electrophoresis Power Pack (Supply), BGPS 300/400, GeNei Merck

7

Cooler Box, GeNei Merck

8

Vortex Mixer, 11-160, GeNei Merck

9

14000rpm Microcentrifuge, SLM-MCF-14K, GeNei

10

Vertical Deepfreezer, BFS-150, Cellfrost

11

Single Channel Micropipette

(a set of 5 with stand), T2,T1000,T20,T10; Tarson

12

Dry bath, LI-DB-120, GeNei Merck

13

Microwave Oven, SHARP

14

UV-VIS Spectrophotometer, Systronics

15

Online UPS with battery & stand, 3KVA (2hr), ELNOVA

16

Silent Generator, 25A5-C, Kirloskar

 

Equipments for Plant tissue culture activities:

Sl. No.

Name of Equipment

1

Refrigerated Liquid Bath inner chamber made of S.S., size 450x300x300mm        

2

Incubator Bacteriological 19”x18”x18” S.S. Chamber

3

Deep Freezer , Ultra Low cabinet 4 cu feet, ambient to  -30

4

Tissue Culture Hood  , size 1200x600x 600mm height at back , S.steel top

5

B.O.D. Incubator [650(W)x580(B)x900(H)mm    

Automatic cyclic Timer  Digital Humidity Controller, Automatic Voltage Stabilizers

6

Shaking Incubator cum BOD   incubator  635x550x550mm,  Digital Speed meter,  & Digital Temp indicator cum Controller with Stabilizers

7

  Laminar Flow Cabinet Horizontal    (SS),    3′x 2′x 2’ UV Light,

  Transparent front door, HEPA filters & PRE filters , Steel Revolving chair,

 Cooling System ( 1.0 tone), Automatic Voltage Stabilizers

8

          Tissue Culture Rack with  Florescence Tubes

 &  5-shelved,  0-24 h Timer, & Stabilizers

9

Distillation Apparatus Glass, (Double Stage)   Accessories – Flask Capacity 5Lit,    Automatic Low water level cut off device.

10

Hot Air Oven    size 455x455x605mm   built in air fan and digital temp. controller

11

  Rotary Flask Shaker (Platform   size 55 x 55 cm)   

  Holding   capacity 36 flask of 100ml, Digital

  Speed meter, Automatic 0-60minute Timer

12

  Autoclave Vertical    350mmx550mm,   Digital   ON/  OFF mechanical device,

Automatic 0-60minute  Timer  ss, Inner busket, Lifting device,  Safety valve,

  Pressure release valve, Water level indicator.

13

Serological Water Bath double walled   SS Chamber Size 605x300x175mm with digital temp. Controller &   High speed stirrer.

14

Test Tube Rotator for 36 tubes

15

Culture Vessels Magnetic  stirring capacity 5 ltr built in Hot plate

16

Tissue Culture Trionocular Microscope with Microphotographic camera kit

17

Micropipettes multi channels Analogue model

18

Electronic Digital Balance , Analytical – Capacity 100gm, Sensitivity 1mg

19

  LCD Projector   “Sony”,    Multimedia Projector – Remote   control ,

   Portable tripod plastic   screen – 60″  x 60″

20

  Computers (Leonova  Flat model) with UPS

  Accessories – Laser jet Printer-1, Inkjet Printer    (1No.), Scanner    (1No.)    

21

Tissue Floatation Bath 200MMX70MM

22

PH Meter (Digital) built in stabilizer     Accessories – Combined glass electrodes  Buffer solution Stand.

23

Microprocessor based pH meter with accessories

24

Water Deioniser   – Four bed Digital Model capacity 100Lit/hr.

25

Tissue Homogenizer , 10000 rpm with speed regulator

26

Tissue Processing Unit with S.S. basket, 24 tissues capsules, with thermostatic controlled wax bath

27

Research Micro Centrifuge (Table Top Model)  with  rotor head carrier for 1x18 (5ml) tubes

28

  Autoclave Portable  300mm x   300mm   Doubled wall  

  With   swing nut type closing lid.   Safety valve,  Pressure release   valve

 

 

 

Major achievements of the Biotech Hub:

The hub is well equipped for carrying out research both on Plant Biotechnology and Molecular Biology. The following Research projects were conducted in the hub.

 

      a)  Plant regeneration by somatic embryogenesis in Azadirachta indica (Neem).

      b)  In vitro micropropagation of Aloe vera, Brahmi, Orchids and Banana.

c) Phytochemical Screening and GC-MS Analysis of Methanolic leaf Extract of an

     Endemic Plant K. assamica.

d) GC-MS Analysis of Bioactive Compounds Present in Methanol Extract of Fruit of An

     Endemic Plant Kayea assamica.

 

e) Identification of medicinally important bioactive compounds present in fruit of endemic

    plant K. assamica for the conservation and sustainable use of the plant species.

 

f) Hepato-Protective Effect of Aqueous Extract of Seed, Leaf and Fruit of Jackfruit

    (Artocarpus heterophyllus Lam.)  Against CCl4 Induced Hepatotoxicity on Swiss

    Albino Mice.

 

g) In vitro germination of Bhut jolokiya from khashi Hills, Meghalaya.

 

h)  Training of Teachers & undergraduate students; arrangement of invited talk, awareness

      /outreach Program, observation of National Science Day, etc.

 

i) Training of UG, PG students and research scholars on handling modern sophisticated

    instruments and equipment in the domain Biotechnology research.

 

j) A number of outreach programs/ invited talks realized to propel sensitization of

    Biotech Research.

 

k) More and more outstation academicians and researchers showing keen interest to

    have association with our Biotech Hub.

 

l) Mentoring Biotech Laboratory at Panigaon and Gogamukh H.S. School under Bliss

   Program of DBT, Govt. of India

 

m) Publication of annual News Bulletin “BIO-NEWS” in the month of April every year.

 

 

Future activities and proposed action plan:

 

  1. To continue research activities (tissue culture & Micropropagation) on medicinal plants
  2. In vitro seed germination of other species of Orchids, Bhut Jalakiya, and Citrus sinensis.
  3. Isolation of endophytes from flower, leaf and root of K. assamica and their chemical and molecular characterization.
  4. Effect of endophytic fungal metabolite in different bioassay (Antimicrobial, anti-larval).
  5. A stepwise scientific work on the endemic medicinal plant K. assamica for its conservation and sustainable use in therapeutic medicine.
  6. To provide UG students in Botany and zoology with exposure to the tools and techniques of Molecular Biology Techniques
  7. Organization of Workshop and Training, Outreach & Awareness Program related to Molecular Biology and Biotechnology.
  8. To provide technical and infrastructure facility support to the faculty members and others.
  9. Capacity building of biology teachers/students of schools/college levels covering the institutes which were not included in last 6 years training/ workshop.
  10. Conducting Awareness/ outreach program in the region targeting school level students and teachers.
  11. More students encourage to take biotechnology based project at under graduate level.

 

 

 

Papers published/ Presented:

 

  1. Plant regeneration by somatic embryogenesis in Azadirachta indicia.Juss (Neem).2017. IRJET Vol.4 (6) Pp. 3212-17 Impact factor 5.181.
  2. Phytochemical Screening and GC-MS Analysis of Methanolic leaf Extract of an Endemic Plant Kayeaassamica. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS). e- ISSN: 2278-3008, p-ISSN: 2319-7676. Volume 12, Issue 5 Ver. II (Sep. – Oct. 2017), PP 07-16; DOI: 10.9790/3008-1205020716.

 

  1. GC-MS Analysis of Bioactive Compounds Present in Methanol Extract of Fruit of An Endemic Plant Kayea assamica.  Presented in IOCBS 2017. IOCBS proceeding ISBN: 978-81-934141-0-1.
  2. Identification of medicinally important bioactive compounds present in fruit of endemic plant Kayea assamica for the conservation and sustainable use of the plant species. Poster presented in Biodiverse 2018, ADNAT Silver Jubilee Convention.
  3. Hepato-Protective Effect of Aqueous Extract of Seed, Leaf and Fruit of Jackfruit (Artocarpus heterophyllus Lam.)  Against CCl4 Induced Hepatotoxicity on Swiss Albino Mice. (Communicated).