Other Programmes

Plant Tissue Culture and Micropropagation :

One year Post B.Sc. Diploma Course in "Plant Tissue Culture and Micropropagation" was introduced in the Department of Botany, North Lakhimpur College, Assam from the session 2008-09. The Course has been sponsored by the Department of Biotechnology, Ministry of Science and Technology, Govt. of India under the Human Resource Development Programme for training technologists in life sciences and biotechnology. Plant tissue culture is the aseptic (free from microorganism) culture of plant cells, tissue or organs on a culture medium under controlled environmental conditions. The techniques are utilized in the field of Biotechnology. A wide range of plants have now been regenerated through technique of tissue culture. The technique is successfully used to conserve germplasm of rare and endangered plant species. It is providing an alternative source for commercial, horticultural and conventional medicinal trade. The Plant tissue culture has an important role for the commercial production of pathogen-free plants. Thus, Plant tissue culture research is a multi- dimensional science that offers exciting prospects to future improvements in crop productivity. Plant tissue culture is also an alternative method for production of useful and valuable secondary metabolites according to demand. Micropropagation is the rapid vegetative propagation of plants through tissue culture techniques. It allows the production of large numbers of plants from small pieces of a mother plant in relatively short periods of time. Practically, in micropropagation, depending on the species any part of a plant is induced to regenerate into complete plant under in vitro condition. The process offer several distinct advantages that are not possible with conventional propagation techniques. Some nurserymen have been introduced to the techniques and advantages of micropropagation. Again some firms have established their own tissue culture facilities and commercial scale operations for the mass productions of ornamental, horticultural and medicinal plants. With the increase in demand for tissue cultured plants, there is an increased demand for experts in plant tissue culture as well as micropropagation. To provide such an opportunity, the department of Botany, North Lakhimpur College, Assam has introduced the course on plant tissue culture and micropropagation with the financial assistance from the Department of Biotechnology, Ministry of Science & Technology, Govt. of India.

Objectives of the course programme :

  • To make the students familiar with basic knowledge and understanding of various aspects of Plant Tissue culture.
  • To teach students the theories, principles and approaches to modern trends in life sciences and plant biotechnology.
  • To help in adapting students to the needs of the tissue culture industry and to enhance their career prospects.
  • To train the students for further education and research.
  • To inspire students for research and development in Plant Biotechnology so that they can activate their hidden potential to enhance their knowledge and skills.
  • To raise awareness among public about the application of plant tissue culture and micropropagation of economically important plant species covering medicinal plants, ornamental plants, horticultural and plantation crops.
  • Infrastructure and other facilities for the programme:

  • Laboratory facilities :Laboratory facilities provided with
  • i) Culture Room

    ii) Working Laboratory

    iii) Inoculation Chamber

    iv) Washing facilities, etc.

  • Lecture Cum Seminar Hall : Lecture room is equipped with audio-visual facilities such as slide projector, LCD Projector, etc.
  • Infrastructural Facilities : Net/ Shade house for hardening along with the medicinal plant garden.
  • Library facilities : Library with well furnished reading room for both students and teachers separately.
  • Common facilities : Computer with internet connectivity, photocopier, printers etc.
  • Major Equipments :

    1. B.O.D. Incubator

    2. Laminar Flow Cabinet

    3.Shaking Incubator cum BOD

    4. Distillation Apparatus

    5. Microprocessor based pH meter

    6. Refrigerated Liquid Bath

    7. Hot Air Oven

    8. Trionocular Microscope

    9. Bacteriological Incubator

    10. Tissue Culture Racks

    11. Rotary Flask Shaker

    12. Autoclave Vertical

    13. Serological Water Bath

    14. Test Tube Rotator

    15. Electronic Digital Balance

    16. Tissue Floatation Bath

    17. Digital pH Meter

    18. Magnetic Stirrer

    19. Tissue Homogenizer

    20. Spectrophotometer

    21. Tissue Culture Hood.

    22. Autoclave Portable

    23. Research Micro centrifuge

    24. Water Deioniser

    25. Micropipette multi channel

    26. Tissue processing Unit

    27. Filter sterilization unit

    28. Glass Bead sterilizer

    Course structure:

    The course consists of two semesters with six papers, all of which must be successfully completed by the students to obtain the Post B.Sc. 'Diploma in Plant Tissue Culture and Micropropagation'.

  • Theory Paper: 200 Marks
  • Practical: 100 Marks

  • Semester-II
  • Theory Paper: 200 Marks
  • Practical: 100 Marks
  • *Theory Class: 12 hrs. / week

    *Practical/Lab. Work: 36 hrs (or more) / week
    Number of seats per session : 15 (Fifteen)
    Studentship : @ Rs. 1200/- pm

    Eligibility for admission:

    Graduate students with Major in Botany/Zoology/Microbiology/Biochemistry/Biotechnology/Chemistry or any other related discipline with at least 45% marks in the main subject or B.Sc. (Gen.) with at least 45% marks in aggregate are eligible to sit in the entrance test. Candidates whose results have not been declared, but are expecting to pass before the entrance test with required norms can also apply.

    Admission procedure:

    Publication of Admission advertisement is made in a local daily (The Assam Tribune/others) as well as in the Employment News. Students are selected on the basis of written test (2hr duration) based on the basic knowledge of Life Sciences and Chemistry. The medium of instruction is English. Central reservation policy is followed in admission.

    How to apply :

    After publication of Admission advertisement, all eligible candidates are required to submit their applications along with complete bio-data mentioning Candidate’s name, Father’s name, Date of birth, Caste, Academic qualification from HSLC onwards, Communication Address, Contact numbers, etc. on or before the date fixed by the college authority.   Candidates can also post their applications electronically as an attachment file to pkmitra94@rediffmail.com. College Admission forms (and also Hostel admission form, if required) will be supplied to the selected candidates before the date of admission. Students have to submit the filled in Admission forms with all essential documents during the time of admission.

    Faculty Members :

  • Core faculty :
  • Name




    Dr. S. N. Phukan

    Dept.of Botany

    Associate Professor


    Dr.(Mrs.) B. Hazarika

    Dept of Botany

    Associate Professor

    Plant Physiology

    Dr. P. K. Mitra 

    Dept of Botany

    Associate Professor

    Cytogenetics and Plant Breeding

    Dr.(Mrs.) R. Phukan

    Dept of Botany

    Asstt. Professor

    Plant Physiology

    Dr. C.R. Bora

    Dept of Botany

    Asstt. Professor

    Plant Taxonomy

    Mr. L. P. Hazarika 

    Dept. of Zoology

    Associate Professor


    Dr. B. Bhuyan 

    Dept of Chemistry

    Asstt. Professor

    Organic Chemistry

    Dr. R.Parajuli

    Dept of Chemistry

    Asstt. Professor

    Physical Chemistry

    Visiting/Collaborating faculty :

    The nearby institutes offering programme with Plant Tissue Culture and Micropropagtion are North East Institute of Science & Technology (NEIST), Jorhat; Dept. of Biotechnology, Assam Agriculture University; Dept. of Biotechnology, IIT Guwahati; Dept. of Molecular Biology & Biotechnology, Tezpur Central University; Dept. of Life Science and Biotechnology, Dibrugarh University; Defence Research Laboratory, Tezpur; Tocklai Tea Experimental Station, Jorhat, Assam; Dept. of Life Sciences, Rajiv Gandhi University; State Forest Research Institute, Itanagar, Arunachal Pradesh, etc. The College shall invite visiting and guest faculties from the above institutes.

    Advisory cum monitoring Committee :

    Prof. A.K. Buragohain

    Dept. of Molecular Biology & Biotechnology,

    Tezpur Central University, Tezpur, Assam


    Defence Research Laboratory,

    Tezpur, Assam

    Dr. A.K. Das

    Professor & Dean, Faculty of Life Sciences,

    Rajiv Gandhi University, Itanagar,

    Arunachal Pradesh.

    Dr. L. Saikia

    Chief Scientist, Regional Agriculture Research Station (AAU),

    North Lakhimpur, Assam

    DBT Nominee

    Dibrugarh University Nominee

    Dr. (Mrs.) Pratibha Sarma

    Specialist, PB & PM,Nagaon Paper Mill,

    Kagajnagar - 782413,Assam

    Dr. S. B. Pando

    Rtd. Principal & Head of the Dept of Botany,

    North Lakhimpur College, Assam

    Dr. B.Boruah


    North Lakhimpur College, Assam

    Dr. S.N. Phukan

    Head, Dept. of Botany,

    North Lakhimpur College, Assam

    Job Prospects :

    Successful completion of the Post B.Sc. Diploma course in Plant Tissue Culture and Micropropagation will facilitate the students:

  • with new job opportunities in R& D departments of government and private sector as a personnel in production unit or as senior technician or technical assistant.
  • To engage themselves in providing consultancy to plant tissue culture industries.
  • With employment opportunities in institutions of higher learning such as university or colleges to assist personnel involved in plant tissue culture.
  • To become self-employed by establishing a small micropropagtion Unit.
  • o develop their own plant tissue culture industry.
  • o take up teaching profession in plant tissue culture.
  • o become an entrepreneur and run large scale plant production unit.
  • Outline of the Practical programme :

    As per DBT's specification, necessary initiatives have been taken for proper training of the students to some Tissue Culture Laboratories, so that students can attain first hand experience and greater exposure. As a part of 1st Semester Syllabus, following practical training were offered to the students in the Tissue Culture Laboratory of North Lakhimpur College, Assam.

  • Technique of glass ware washing and cotton plug preparation.
  • Operation of different major equipments needed for plant tissue culture.
  • Preparation of stock solution and culture medium.
  • Explant preparation, methods of sterilization and inoculation.
  • Cultures of shoot, root, leaf, nodal portion, shoot tip, embryo, seed etc. of some selected plant species.
  • Callus culture, sub-culture and regeneration.
  • Method of synthetic seed production.
  • In the Second Semester students will have to submit project report on some selected topics as part of their practical works. Likely, students are divided into 4/5 groups to perform project work. Selected topics are Micropropagation of Orchids (Ornamental plant), Tissue culture and micropropagation of Neem/Agave/ Brahmi (Medicinal plants) and micropropagtion of Bamboo/ banana (Plantation crop) etc. In project work students have to perform culture, sub-culture, regeneration and transfer to the soil of the selected plant species.

    Contact Address :
    Dr. Pradip Kr. Mitra, Course Coordinator,
    Dept of Botany, North Lakhimpur College,
    Khelmati-787031, Lakhimpur, Assam, INDIA
    Mobile : 09435084586, e-mail : pkmitra94@rediffmail.com , Fax: 03752-232234
    North Lakhimpur College
    Khelmati, Lakhimpur-787031